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How to get through the laser wall without turning
In the level "escape from oscorp" swing to the right hall and into the room with the switch for the gun turrets. Instead of going back to the left hall to run off the laser wall stay in the same room and impact web the windows. They will break and you can go down without having to go through the pain of turning off the laser wall. From there continue normally. This also works with green goblin and shocker. With green goblin use any bomb or gun or the sharp things to break the windows. With shocker use L + Y to zap the windows and continue normally.
No Robots searching the base
In the part where you have to wait for Dr. Rue to get the keycard for lab D in the level "chemical chaos". Stay on the roof near Lab D and when he comes shoot him with Impact webbing before he says...."there is an intruder, spread out and search". IF you shoot him before he will not say that and the robots will not be searching for you and also the alarm will not go off.
Secret Web Combo
Okay, after you acquire the Adv. Impact Webbing combo, hold L +Y for 5 seconds then let go. Spidey should throw out a HUGE, bigger version than the normal impact webbing. This webbing is very strong and can kill anyone (or anything) very easily...