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Harder Version of Final Boss
Normally, the final boss fight is pretty simple-it only takes 5 super missile hits to kill him, if you target the core.
However, if you achieve a 100% item collection rate, the boss will take 15 super missiles to kill, and will do 50% more damage, which varies with your difficulty level. So, if you have 100% on hard mode, the boss will take out three tanks with one swipe of his claw, instead of two.
Open New Time Attack mode
To open Time Attack Mode in which you get a password for your clear time and percent, choose a file that has the green+yellow Samus symbol and hit the A button. When you see the 'Start Game' option, simply hit Up, Left, Right, then Down on the d-pad and 'New Time Attack' will appear.